Reporter

Part:BBa_K216014:Design

Designed by: Edinburgh iGEM 2009   Group: iGEM09_Edinburgh   (2009-10-16)


PyeaR with Renilla luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 671


Design Notes

No special considerations. Sequencing suggests that there is an extra 'A' base following the scar between the RBS and the Renilla luciferase; that is, the scar region reads TACTAGAATG instead of TACTAGATG. If the sequencing is correct, this base must be present in J52008 or have been introduced by a random event during the construction process. This makes the gap between the RBS and start codon one base longer, but still well within the optimal range for E. coli and B. subtilis.


Source

Made by joining PyeaR BioBrick BBa_K216005 to a PCR fusion of strong synthetic ribosome binding site J15001 and Renilla luciferase BioBrick J52008.

References